Preliminary studies on the vitrification of red sea bream (Pagrus major) embryos. Resfriamento de embriões de peixes neotropicais In: AQUACIÊNCIA, Palmas-TO, 2012.ĭING F.H. Cryopreservation of flounder (Paralichthys olivaceus) embryos by vitrification. Cryopreservation of late embryos and early larvae in the oyster and hard clam. Preliminary studies on the cryopreservation of gilthead seabream (Sparus aurata) embryos. Effect of different cryoprotectants and vitrificant solutions on the hatching rate of turbot embryos (Scophthalmus maximus). Cryoprotectant microinjection toxicity and chilling sensitivity in gilthead seabream (Sparus aurata) embryos. Major vitelline envelope proteins in piperfish oocytes originate within the follicle and are associated with the Z3 layer. World Aquaculture Society, Baton Rouge, 2000. New approaches in cryopreservation of fish embryos, In: CRYOPRESERVATION IN AQUATIC SPECIES. Development, Growth and Differentiation, v. Cryopreservation of sea urchin embryos and sperm. Stage-dependent hatching responses of rohu (Labeo rohita) embryos to different concentrations of cryoprotectants and temperatures. The hatching of common carp (Cyprinus carpio L.) embryos in response to exposure to different concentrations of cryoprotectant at low temperatures. brachypomus embryos at the optical vesicle appearance stage (13 hpf) should be chilled in a solution containing 17.5% glucose and 10% methanol for up to eight six at 2☌.ĪHAMMAD M.M. In general, larvae chilled at a more developed stages (8 and 13 hpf), in methanol and for shorter periods could survive chilling and develop normally, compared to the other treatments. The highest percentage of normal and live larvae (30.6%) was observed when embryos were chilled at 13-hpf in methanol for 6 hours. The total number of treatments was 4 stages x 3 cryoprotectants x 4 periods of chilling. One hundred embryos were selected from each of the four stages and chilled in methanol, methylglycol or dimethylsulfoxide (DMSO) for 6, 8, 10 or 12 hours, at 2✬. Embryos at the following ontogenetic stages were used: blastoderm – 1.2 hours post-fertilization (hpf) epiboly – 5 hpf blastopore closure – 8 hpf and appearance of optic vesicle – 13 hpf. The aim of this study was to chill Piaractus brachypomus embryos at different stages of development in some cryoprotectants and for various periods of chilling. Cryopreservation has not been successfully used to preserve fish embryos, although chilling techniques have been used with good results.
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